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stopped flow spectrophotometer  (Applied Photophysics)


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    Applied Photophysics stopped flow spectrophotometer
    Stopped Flow Spectrophotometer, supplied by Applied Photophysics, used in various techniques. Bioz Stars score: 99/100, based on 3404 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stopped flow spectrophotometer/product/Applied Photophysics
    Average 99 stars, based on 3404 article reviews
    stopped flow spectrophotometer - by Bioz Stars, 2026-03
    99/100 stars

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    Applied Photophysics stopped flow instrument rx2000 rapid kinetics
    GLOD4 accelerates the decomposition of peroxynitrite. ( A <t>)</t> <t>Stopped-flow</t> spectroscopy of peroxynitrite decomposition at 302 nm. Typical tracings for the initial 2 s in the absence (black) or presence (gray) of 25 µM GLOD4 are shown. ( B ) Observed rate constant determined from fitting time courses collected for various initial mixing concentrations of peroxynitrite to the exponential function A[1 − Exp(−kobs*t)]. ( C ) Kinetics of peroxynitrite decomposition monitored by stopped-flow mixing of varying concentrations of peroxynitrite in 0.05 M NaOH or peroxynitrite in 0.05 M NaOH with 25 µM GLOD4 in 0.5 M Tris pH 8.0.
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    GLOD4 accelerates the decomposition of peroxynitrite. ( A ) Stopped-flow spectroscopy of peroxynitrite decomposition at 302 nm. Typical tracings for the initial 2 s in the absence (black) or presence (gray) of 25 µM GLOD4 are shown. ( B ) Observed rate constant determined from fitting time courses collected for various initial mixing concentrations of peroxynitrite to the exponential function A[1 − Exp(−kobs*t)]. ( C ) Kinetics of peroxynitrite decomposition monitored by stopped-flow mixing of varying concentrations of peroxynitrite in 0.05 M NaOH or peroxynitrite in 0.05 M NaOH with 25 µM GLOD4 in 0.5 M Tris pH 8.0.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Selective peroxynitrite-mediated protein nitration catalyzed by glyoxalase domain containing protein 4

    doi: 10.1073/pnas.2515002123

    Figure Lengend Snippet: GLOD4 accelerates the decomposition of peroxynitrite. ( A ) Stopped-flow spectroscopy of peroxynitrite decomposition at 302 nm. Typical tracings for the initial 2 s in the absence (black) or presence (gray) of 25 µM GLOD4 are shown. ( B ) Observed rate constant determined from fitting time courses collected for various initial mixing concentrations of peroxynitrite to the exponential function A[1 − Exp(−kobs*t)]. ( C ) Kinetics of peroxynitrite decomposition monitored by stopped-flow mixing of varying concentrations of peroxynitrite in 0.05 M NaOH or peroxynitrite in 0.05 M NaOH with 25 µM GLOD4 in 0.5 M Tris pH 8.0.

    Article Snippet: Then, 50 μM GLOD4 in 1.0 M Tris (pH 8.0) was loaded into one syringe of an SX.20 stopped-flow fluorometer (Applied Photophysics, Letherhead, UK).

    Techniques: Spectroscopy