Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Selective peroxynitrite-mediated protein nitration catalyzed by glyoxalase domain containing protein 4
doi: 10.1073/pnas.2515002123
Figure Lengend Snippet: GLOD4 accelerates the decomposition of peroxynitrite. ( A ) Stopped-flow spectroscopy of peroxynitrite decomposition at 302 nm. Typical tracings for the initial 2 s in the absence (black) or presence (gray) of 25 µM GLOD4 are shown. ( B ) Observed rate constant determined from fitting time courses collected for various initial mixing concentrations of peroxynitrite to the exponential function A[1 − Exp(−kobs*t)]. ( C ) Kinetics of peroxynitrite decomposition monitored by stopped-flow mixing of varying concentrations of peroxynitrite in 0.05 M NaOH or peroxynitrite in 0.05 M NaOH with 25 µM GLOD4 in 0.5 M Tris pH 8.0.
Article Snippet: Then, 50 μM GLOD4 in 1.0 M Tris (pH 8.0) was loaded into one syringe of an SX.20 stopped-flow fluorometer (Applied Photophysics, Letherhead, UK).
Techniques: Spectroscopy